NUCLISENS® MINIMAG®
The straightforward answer for manual nucleic acid extraction
- Compact unit (43.8 x 11.4 x 15.3 cm’s) can fit on benchtop or in hood
- Leverages bioMérieux expertise of gold standard BOOM technology
- Generic extraction of DNA & RNA from a variety of specimen types and volumes simultaneously
- Description
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- TECHNICAL DETAILS
Description
NUCLISENS®MINIMAG®instrument is the NUCLISENS® family’s compact manual solution for premium quality total nucleic acid extraction. Both efficient and convenient, it uses the enhanced magnetic silica version of BOOM technology, a gold standard for universal extraction of RNA and DNA.
Go Boom
BOOM technology, first patented by bioMérieux, is a gold standard for the universal extraction of RNA and DNA. Based on the ability of silica to bind DNA and RNA in high salt concentrations, BOOM’s leading performance is recognized in a number of papers and comparative evaluations*. bioMérieux’s in-house expertise of this technology sets our extraction chemistry apart.
*For example, Rutjes et al. showed that with the MINIMAG® “RNA levels increased at least 100 to 500 times” (Rutjes et al. , AEM,(71), 7, 2005, pp 3734-3740). Tang et al. showed that “MINIMAG® produced the highest quantity of nucleic acids and the best precision amongst the three systems [tested]”. (Tang et al., JCM, (43), 9, 2005, pp4830-4833).
Quality extraction
Our high affinity magnetic silica, combined with further optimization of the extraction buffers, significantly enhances the quality of the nucleic acid extraction.
- Very efficient washing & collection of magnetic silica
- Enhanced concentration with eluate volume as low as 25 μl
- Increased weight-to-volume ratio of new magnetic silica results in exceptional DNA/RNA recovery
- No use of ethanol or other organic solvents
- Highly effective removal of inhibitors
- Excellent purities as shown by OD260/OD280 ratio *
- No apparent degradation (see diagram*)
Move downstream efficiently
NUCLISENS® MINIMAG®conveniently fits in any lab. With a small footprint (43.8 x 11.4 x 15.3 cm’s), it can be used on a benchtop or in the hood. As a manual extractor, its smart design offers efficiency to move downstream fast:
- Use a single set of reagents to simultaneously process various sample types and volumes
- Specify different elution volumes within a single run
- Up to 12 extractions can performed with one instrument in less than 1h
- With 2 NUCLISENS® MINIMAG® units, 24 samples can be extracted in 90 minutes
- Eluate is ready for immediate use on downstream applications
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Instrument Specifications
Weight (uncrated) 3.6 kg (8 lbs)
Dimensions: Length 43.8 cm (17.25”) x Width 11.4 cm (4.5”) x Height 15.3 cm (6”)
Working Volumes:
Minimum 50µl
Maximum 1000 μl
Operating Temperature: 4 to 45 °C
Power Requirement: 24 volts DC desktop switching
Power supply at:
Europe: 240 Volts AC 50/60Hz
USA and Canada: 110 Volts AC 60Hz
Consult your local bioMérieux representative for product availability
SERVICE & SUPPORT
Examples of applications:
- Target: HIV-RNA,
- Sample Type: Plasma
- Target: HBV DNA
- Sample Type: Serum
- Target: Enterovirus RNA
- Sample Type: CSF, Stool, Throat swab
- Target: CMV mRNA
- Sample Type: Whole blood
- Target: Human mRNA (U1A)
- Sample Type: Sputum, Whole blood
- Target: Mycobacterium tuberculosis RNA
- Sample Type: Sputum
- Target: Mycobacterium tuberculosis DNA
- Sample Type: Sputum
- Target: Legionella pneumophila RNA
- Sample Type: Lung biopsy
- Target: Bordetella pertussis RNA
- Sample Type: Gargle
- Target: Mycoplasma pneumoniae RNA
- Sample Type: Gargle, Throat swab
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